Build Cap 0 structures found on the 5' end of most eukaryotic mRNA with better confidence than co-transcriptional capping tools

The capping of in vitro transcribed RNA improves the stability and in vivo translation efficiency of transfected mRNA in most eukaryotic cells. Capped mRNA is also more efficiently translated in some in vitro translation systems. EPICENTRE’s ScriptCap™ m⁷G Capping System builds the Cap 0 structure found on the 5' end of most eukaryotic mRNA molecules. Based upon the tri-functional Vaccinia Virus capping enzyme (mRNA triphosphatase, guanylyltransferase and guanine-7-methyltransferase), this system includes all of the components necessary to convert RNA containing a 5' triphosphate to Cap 0 RNA. Unlike other co-transcriptional capping systems which have theoretical capping yields of less than 80% capped message, the ScriptCap™ m⁷G Capping System is a convenient, excellent value, highly efficient, single-enzyme capping system, which is ideally suited for quantitative production of Cap 0-capped RNA.