Easy, Reliable Isolation of High-Quality BAC DNA
EPICENTRE's BACMAX™ DNA Purification Kit enables easy, reliable isolation of exceptionally pure intact BAC DNA without binding columns, resins, or organic extractions. The high-quality BAC DNA is suitable for many applications, including sequencing, fingerprinting, PCR, and preparation of shotgun libraries. The scalable protocol is based on a modified alkaline-lysis procedure that typically yields 0.6 to 25 µg of BAC DNA from a 1.5 to 100 ml culture of a single-copy BAC. Selective precipitation steps and the incorporation of EPICENTRE's RiboShredder™ RNase Blend remove contaminants that degrade DNA and interfere with downstream applications.
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